Device

Part:BBa_J100202:Design

Designed by: Malcolm Campbell   Group: Campbell M Lab   (2014-11-21)


Intermediate for IndClone Red


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 805
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 805
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 805
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 805
    Illegal AgeI site found at 678
    Illegal AgeI site found at 790
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

No BsaI sites so we will be able to later use Golden Gate Assembly.


Source

E. coli-based genes

References